Abstract. This article tests contextual and individual-level explanations of the gender gap in political knowledge in Latin American countries. It suggests that this gap is impacted by political and economic settings through two interrelated mechanisms: gender accessibility (that is, the extent of available opportunities for women to influence the political agenda) and gender-bias signaling (that is, the extent to which women play important roles in the public sphere). Analyzing data from the 2008 Americas Barometer survey, this study shows that the gender gap in political knowledge is smaller among highly educated citizens, in rural areas (where both men and women know little about politics) and in bigger cities (where women’s levels of political knowledge are higher). More importantly, the magnitude of the gap varies greatly across countries. Gender differences in income, party system institutionalization and the representation of women in national parliaments are all found to play a particularly important role in explaining the magnitude of the gender gap in political knowledge across Latin America.